Differential staining of ocular goblet cells

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Novel Approach of Differential Staining to Detect Necrotic Cells in Preimplantation Embryos

Background This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst. MaterialsAndMethods Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidium iodide (PI; 300μg/ml) and bisbenzimide (Hoechst: H33342; 5μg/ml) fluorescent dyes. Embryos were th...

متن کامل

Goblet Cells Contribute to Ocular Surface Immune Tolerance—Implications for Dry Eye Disease

Conjunctival goblet cell (GC) loss in dry eye is associated with ocular surface inflammation. This study investigated if conjunctival GCs contribute to ocular surface immune tolerance. Antigens applied to the ocular surface, imaged by confocal microscopy, passed into the conjunctival stroma through goblet cell associated passages (GAPs) in wild type C57BL/6 (WT), while ovalbumin (OVA) was retai...

متن کامل

Goblet cell loss abrogates ocular surface immune tolerance.

Intestinal epithelial cells condition tolerogenic properties in DCs. Aqueous-deficient dry eye is associated with goblet cell (GC) loss and increased IFN-γ expression in the conjunctiva. We hypothesized that loss of GCs reduces tolerance-inducing properties of antigen presenting cells (APCs) in the conjunctiva and draining nodes. Mice lacking the SAM pointed domain containing ETS transcription ...

متن کامل

Differential staining of neuronal and glial nuclei.

The histological differentiation of neuronal from glial nuclei may be useful in the identification of tumours and in the study of developing nervous tissues. The following method does this by staining the glial nucleus red and the neuronal nucleus green. It also stains other structures in the neural parenchyma. Formol-fixed tissues taken down to water and embedded in paraffin are used. 1 The se...

متن کامل

Quantitative Studies of Differential Staining Reactions

It has been shown previously (Kennedy and Barbaro, 1953) that the amount of crystal violet adsorbed by bacteria can be measured quantitatively by either one of two procedures. One procedure involved micro-Kjeldahl analyses of bacterial cells before and after staining. The increase in total nitrogen in the stained cells represented the dye nitrogen adsorbed. The second procedure was based on mic...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Eye

سال: 1989

ISSN: 0950-222X,1476-5454

DOI: 10.1038/eye.1989.128